A highly reproducible measurement of airway and vascular reactivity
A powerful tool to measure airway and vascular reactivity is the human precision cut lung slice (PCLS). To quantify underlying changes, we describe a simpler, faster, and highly-sensitive approach called tissue traction microscopy (TTM). Applicable to asthma, COPD, and PAH simulated tissues.
How does TTM work?
A) We adhere the PCLS upon a custom elastic substrate prepared in a single- or a multi-well formats. B) PCLS adhesion does not affect airway reactivity. C) Representative bright-field images and overlaid contraction maps in response to 10−4 M ACh (30 min) and 10−3 M chloroquine (ChQ; 25 min). Scale bar = 500 μm. D) and E): Time-dependent lumen area changes (gray line) and airway contraction changes (black line) in response to 10−4 M ACh followed by 10−3 M ChQ. Compared to lumen airway changes, airway contraction measurements are less variable from airway-to-airways,
Ram-Mohan et al, Am J Physiol Lung Cell Mol Physiol. 2020 Feb 1;318(2):L323-L330.
Statistics
Small
Set-up Cost
<5 mins
Per PCLS
Multi-well
1, 6, 12 or 24 well format
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